| 敏感性 | 對熱敏感、易吸潮 |
| 存貯條件 | 儲存溫度-20°C，充氬保存 |
| 應(yīng)用 | Applications for enzyme-labeled (HRP, AP) streptavidin:Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodiesCell and tissue staining (for light microscopy)Western blotting (WB) and other membrane-based biotin detectionApplications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin:Immunofluorescent assays (IFA)Tissue staining (for fluorescence microscopy)Blot immunostainingCell staining (incl. fluorescent activated cell sorting) |
| 產(chǎn)品介紹 | Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups.These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. |
| 備注 | 單位定義：1單位結(jié)合1.0 UG 生物素. 方法描述見文獻(xiàn) GREEN N.M., METHODS IN ENZYMOLOGY, 18, 418 (1970).來源：Streptomyces avidinii |
| 生化機理 | reptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. OursStreptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation. |
| 別名 | 抗生物素蛋白鏈菌素，鏈親合素;Streptavidin 來源于阿維丁鏈霉菌;Streptavidin from Streptomyces avidinii;Streptavidin and Conjugates |
乳膠/丁腈手套 離心管 酵母粉 蛋白胨 中藥對照品 Elisa試劑盒 醫(yī)藥中間體
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