|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Rat
|
64389
|
O88514
|
E. coli
|
41aa
|
4.4kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Rat BD-4 (Recombinant Rat β-defensin 4; rRtBD-4)；重組大鼠β-防御素4
|
|
Synonyms
|
Beta-defensin 4; BD-4; BD-2; Defensin, beta 4; RBD-2; RBD-4; Defb4; Defb2
|
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 10mM PB, pH7.4, 500mM NaCl.
|
|
Endotoxin
|
Less than 1EU/μg of rRtBD-4 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.
|
|
Amino Acid Sequence
|
QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
P6438-5μg
|
Recombinant Rat BD-4
|
5μg
|
|
P6438-20μg
|
Recombinant Rat BD-4
|
20μg
|
|
P6438-100μg
|
Recombinant Rat BD-4
|
100μg
|
|
P6438-1mg
|
Recombinant Rat BD-4
|
1mg
|
|
-
|
說明書
|
1份
|
保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以首次配制成相應濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結(jié)合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
黨沛 
