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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
|
|
Mouse
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50929
|
Q9JJY9
|
E. coli
|
146aa
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16.6kDa
|
－
|
|
About this Antibody
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Name
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Recombinant Murine IL-22 (Recombinant Murine Interleukin-22; rMuIL-22)；Recombinant Mouse IL-22 ; 重組小鼠白細(xì)胞介素22
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|
Synonyms
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IL-TIF; Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL-22IL21; IL-D110; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18; ZCYTO18
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|
Purity
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>96% by SDS-PAGE and HPLC analyses.
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|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.2ng/ml, corresponding to a specific activity of >5.0×106IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH7.4.
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Endotoxin
|
Less than 1EU/μg of rMuIL-22 as determined by LAL method.
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|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
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Category
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Cytokine
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|
Background
|
IL-22 is belonging to IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes and dendritic cells, IL-10 contributes to the inflammatory response in vivo. IL-22 signals through CRF2-4 and IL-22. It along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Recombinant mouse interleukin-22 contains 147 amino acids and it has approximately 81% and 92% amino acid sequence identity with human and rat IL-22.
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|
Amino Acid Sequence
|
LPVNTRCKLE VSNFQQPYIV NRTFMLAKEA SLADNNTDVR LIGEKLFRGV SAKDQCYLMK QVLNFTLEDV LLPQSDRFQP YMQEVVPFLT KLSNQLSSCH ISGDDQNIQK NVRRLKETVK KLGESGEIKA IGELDLLFMS LRNACV
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
P5961-2μg
|
Recombinant Murine IL-22
|
2μg
|
|
P5961-10μg
|
Recombinant Murine IL-22
|
10μg
|
|
P5961-100μg
|
Recombinant Murine IL-22
|
100μg
|
|
P5961-1mg
|
Recombinant Murine IL-22
|
1mg
|
|
-
|
說明書
|
1份
|
保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以首次配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點，使細(xì)胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的安全和健康，請穿實驗服并戴一次性手套操作。
黨沛 
