概述
Recombinant Human/Mouse Fibroblast Growth Factor 8B is produced by our E.coli expression system and the target gene encoding Gln23-Arg215 is expressed.
使用說(shuō)明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技術(shù)規(guī)格
| Tag | None |
| 種屬 | Human/Mouse |
| 表達(dá)系統(tǒng) | E.coli |
| Accession# | P55075-3/P37237-2 |
| Source | E.coli |
| Formulation_Description | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Storage | Lyophilized protein should be stored at < -20°C, thoμgh stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Reconstitution | Dissolve the lyophilized protein in distilled water. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Background | Fibroblast growth factor 8 (FGF-8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen--dependent growth of mouse mammary carcinoma cells. Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial--mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
| Biological_Activity | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 10-60 ng/ml. |
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