概述
Recombinant Human 5-formyltetrahydrofolate Cyclo-ligase is produced by our E.coli expression system and the target gene encoding Met1-Ala203 is expressed with a 6His tag at the C-terminus.
使用說明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技術規(guī)格
| Tag | C-6His |
| 種屬 | Human |
| 表達系統(tǒng) | E.coli |
| Accession# | P49914 |
| Source | E.coli |
| Formulation_Description | Supplied as a 0.2 μm filtered solution of 20mM Tris, 200mM Nacl, 1mM DTT, 50% Glycerol, pH 8.0. |
| Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
| Background | 5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. |
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