概述
Recombinant Human Ubiquitin-conjugating Enzyme E2 D3 is produced by our E.coli expression system and the target gene encoding Met1-Met147 is expressed.
使用說明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技術(shù)規(guī)格
Tag | None |
種屬 | Human |
表達(dá)系統(tǒng) | E.coli |
Accession# | P61077 |
Source | E.coli |
Formulation_Description | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
Purity | Greater than 90% as determined by reducing SDS-PAGE. |
Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
Background | UBE2D3 is an enzyme that belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. |